Functional metagenomics to study antibiotic resistance

Manish Boolchandani, Sanket Patel, Gautam Dantas

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-329
Number of pages23
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1520
ISSN (Print)1064-3745

Keywords

  • Antibiotic resistance genes
  • Functional metagenomics
  • Functional selections
  • High-throughput assembly
  • Massively parallel DNA sequencing
  • PARFuMS
  • Profile HMM-based annotation
  • Resfams
  • Resistome

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  • Cite this

    Boolchandani, M., Patel, S., & Dantas, G. (2017). Functional metagenomics to study antibiotic resistance. In Methods in Molecular Biology (pp. 307-329). (Methods in Molecular Biology; Vol. 1520). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6634-9_19