TY - CHAP
T1 - Functional metagenomics to study antibiotic resistance
AU - Boolchandani, Manish
AU - Patel, Sanket
AU - Dantas, Gautam
N1 - Funding Information:
This work was supported in part by the NIH Director’s New Innovator Award (http://commonfund.nih.gov/newinnovator/), the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK:http://www.niddk.nih.gov/), and the National Institute of General Medical Sciences (NIGMS: http://www. nigms.nih.gov/), of the National Institutes of Health (NIH) under award numbers DP2DK098089 and R01GM099538to G.D. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.
Publisher Copyright:
© Springer Science+Business Media New York 2017.
PY - 2017
Y1 - 2017
N2 - The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
AB - The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
KW - Antibiotic resistance genes
KW - Functional metagenomics
KW - Functional selections
KW - High-throughput assembly
KW - Massively parallel DNA sequencing
KW - PARFuMS
KW - Profile HMM-based annotation
KW - Resfams
KW - Resistome
UR - http://www.scopus.com/inward/record.url?scp=84996605738&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6634-9_19
DO - 10.1007/978-1-4939-6634-9_19
M3 - Chapter
C2 - 27873261
AN - SCOPUS:84996605738
T3 - Methods in Molecular Biology
SP - 307
EP - 329
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -