Functional genomic analysis of RNA interference in C. elegans

John K. Kim, Harrison W. Gabel, Ravi S. Kamath, Muneesh Tewari, Amy Pasquinelli, Jean François Rual, Scott Kennedy, Michael Dybbs, Nicolas Bertin, Joshua M. Kaplan, Marc Vidal, Gary Ruvkun

Research output: Contribution to journalArticlepeer-review

110 Scopus citations


RNA interference (RNAi) of target genes is triggered by double-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for RNAi, we performed a genome-wide screen using an engineered RNAi sensor strain of Caenorhabditis elegans. The RNAi screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/processing factors, chromatin-associated factors, DNA recombination proteins, nuclear import/export factors, and 11 known components of the RNAi machinery. We demonstrate that some of these genes are also required for germline and somatic transgene silencing. Moreover, the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways.

Original languageEnglish
Pages (from-to)1164-1167
Number of pages4
Issue number5725
StatePublished - May 20 2005


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