Functional g protein-coupled receptors on nuclei from brain and primary cultured neurons

Yuh Jiin I. Jong, Karen L. O’malley

Research output: Contribution to journalArticle

Abstract

A growing number of G protein-coupled receptors (GPCRs) have been identifi ed on nuclear membranes. In many cases, it is unknown how the intracellular GPCR is activated, how it is traffi cked to nuclear membranes, and what long-term signaling consequences follow nuclear receptor activation. Here we describe how to isolate nuclei that are free from plasma membrane and cytoplasmic contamination yet still exhibit physiological properties following receptor activation.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalMethods in Molecular Biology
Volume1234
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Calcium imaging
  • GPCR
  • Immunoblotting
  • Metabotropic glutamate receptor
  • Nuclear preparation
  • mGluR5

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