A growing number of G protein-coupled receptors (GPCRs) have been identifi ed on nuclear membranes. In many cases, it is unknown how the intracellular GPCR is activated, how it is traffi cked to nuclear membranes, and what long-term signaling consequences follow nuclear receptor activation. Here we describe how to isolate nuclei that are free from plasma membrane and cytoplasmic contamination yet still exhibit physiological properties following receptor activation.
- Calcium imaging
- Metabotropic glutamate receptor
- Nuclear preparation