@article{256416059d8e4917aada8687d7ee1aa0,
title = "Functional dissection of the ARGONAUTE7 promoter",
abstract = "ARGONAUTES are the central effector proteins of RNA silencing which bind target transcripts in a small RNA-guided manner. Arabidopsis thaliana has 10 ARGONAUTE (AGO) genes, with specialized roles in RNA-directed DNA methylation, post-transcriptional gene silencing, and antiviral defense. To better understand specialization among AGO genes at the level of transcriptional regulation we tested a library of 1497 transcription factors for binding to the promoters of AGO1, AGO10, and AGO7 using yeast 1-hybrid assays. A ranked list of candidate DNA-binding TFs revealed binding of the AGO7 promoter by a number of proteins in two families: the miR156-regulated SPL family and the miR319-regulated TCP family, both of which have roles in developmental timing and leaf morphology. Possible functions for SPL and TCP binding are unclear: we showed that these binding sites are not required for the polar expression pattern of AGO7, nor for the function of AGO7 in leaf shape. Normal AGO7 transcription levels and function appear to depend instead on an adjacent 124-bp region. Progress in understanding the structure of this promoter may aid efforts to understand how the conserved AGO7-triggered TAS3 pathway functions in timing and polarity.",
keywords = "Argonaute, leaf development, leaf polarity, post-transcriptional regulation, transcriptional regulation",
author = "Hoyer, {J. Steen} and Pruneda-Paz, {Jose L.} and Ghislain Breton and Hassert, {Mariah A.} and Holcomb, {Emily E.} and Halley Fowler and Bauer, {Kaylyn M.} and Jacob Mreen and Kay, {Steve A.} and Carrington, {James C.}",
note = "Funding Information: We thank Danforth Center Plant Growth Facility staff members for excellent plant care, G. Nguyen and R. Allscheid for logistical support, and members of the Carrington lab for helpful discussions. D.H. Chitwood and J.G. Hodge provided essential advice on histological analysis. We thank T.C. Mockler and members of his lab (J. Gierer, D. O'Brien, M. Wiechert) for assistance with their plate readers and liquid‐handling equipment. This work was supported by US National Science Foundation award 1330562 (to JCC) and US National Institute of Health grants AI043288 (to JCC), GM056006 (to SAK and JLP‐P), and GM067837 (to SAK). JSH was supported by an NSF graduate research fellowship (award 1143954). Funding Information: US National Science Foundation award, Grant/Award Number: 1330562; US National Institute of Health grants, Grant/ Award Number: AI043288, GM056006, GM067837; NSF graduate research fellowship award, Grant/Award Number: 1143954 Funding Information: We thank Danforth Center Plant Growth Facility staff members for excellent plant care, G. Nguyen and R. Allscheid for logistical support, and members of the Carrington lab for helpful discussions. D.H. Chitwood and J.G. Hodge provided essential advice on histological analysis. We thank T.C. Mockler and members of his lab (J. Gierer, D. O'Brien, M. Wiechert) for assistance with their plate readers and liquid-handling equipment. This work was supported by US National Science Foundation award 1330562 (to JCC) and US National Institute of Health grants AI043288 (to JCC), GM056006 (to SAK and JLP-P), and GM067837 (to SAK). JSH was supported by an NSF graduate research fellowship (award 1143954). Publisher Copyright: {\textcopyright} 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.",
year = "2019",
month = jan,
doi = "10.1002/pld3.102",
language = "English",
volume = "3",
journal = "Plant Direct",
issn = "2475-4455",
number = "1",
}