TY - JOUR
T1 - Functional comparison of RGS9 splice isoforms in a living cell
AU - Martemyanov, Kirill A.
AU - Krispel, Claudia M.
AU - Lishko, Polina V.
AU - Burns, Marie E.
AU - Arshavsky, Vadim Y.
PY - 2008/12/30
Y1 - 2008/12/30
N2 - Two isoforms of the GTPase-activating protein, regulator of G protein signaling 9 (RGS9), control such fundamental functions as vision and behavior. RGS9-1 regulates phototransduction in rods and cones, and RGS9-2 regulates dopamine and opioid signaling in the basal ganglia. To determine their functional differences in the same intact cell, we replaced RGS9-1 with RGS9-2 in mouse rods. Surprisingly, RGS9-2 not only supported normal photoresponse recovery under moderate light conditions but also outperformed RGS9-1 in bright light. This versatility of RGS9-2 results from its ability to inactivate the G protein, transducin, regardless of its effector interactions, whereas RGS9-1 prefers the G protein-effector complex. Such versatility makes RGS9-2 an isoform advantageous for timely signal inactivation across a wide range of stimulus strengths and may explain its predominant representation throughout the nervous system.
AB - Two isoforms of the GTPase-activating protein, regulator of G protein signaling 9 (RGS9), control such fundamental functions as vision and behavior. RGS9-1 regulates phototransduction in rods and cones, and RGS9-2 regulates dopamine and opioid signaling in the basal ganglia. To determine their functional differences in the same intact cell, we replaced RGS9-1 with RGS9-2 in mouse rods. Surprisingly, RGS9-2 not only supported normal photoresponse recovery under moderate light conditions but also outperformed RGS9-1 in bright light. This versatility of RGS9-2 results from its ability to inactivate the G protein, transducin, regardless of its effector interactions, whereas RGS9-1 prefers the G protein-effector complex. Such versatility makes RGS9-2 an isoform advantageous for timely signal inactivation across a wide range of stimulus strengths and may explain its predominant representation throughout the nervous system.
KW - G proteins
KW - Phototransduction
KW - RGS proteins
UR - http://www.scopus.com/inward/record.url?scp=58549091647&partnerID=8YFLogxK
U2 - 10.1073/pnas.0808941106
DO - 10.1073/pnas.0808941106
M3 - Article
C2 - 19098104
AN - SCOPUS:58549091647
SN - 0027-8424
VL - 105
SP - 20988
EP - 20993
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 52
ER -