Functional characterization of a prokaryotic Kir channel

Decha Enkvetchakul, Jaya Bhattacharyya, Iana Jeliazkova, Darcy K. Groesbeck, Catherine A. Cukras, Colin G. Nichols

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The Kir gene family encodes inward rectifying K+ (Kir) channels that are widespread and critical regulators of excitability in eukaryotic cells. A related gene family (KirBac) has recently been identified in prokaryotes. While a crystal structure of one member, KirBac1.1, has been solved, there has been no functional characterization of any KirBac gene products. Here we present functional characterization of KirBac1.1 reconstituted in liposomes. Utilizing a 86Rb+ uptake assay, we demonstrate that KirBac1.1 generates a K+-selective permeation path that is inhibited by extraliposomal Ba2+ and Ca2+ ions. In contrast to KcsA (an acid-activated bacterial potassium channel), KirBac1.1 is inhibited by extraliposomal acid (pKa ∼ 6). This characterization of KirBac1.1 activity now paves the way for further correlation of structure and function in this model Kir channel.

Original languageEnglish
Pages (from-to)47076-47080
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number45
DOIs
StatePublished - Nov 5 2004

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