TY - JOUR
T1 - Functional characterization of a panel of high-grade serous ovarian cancer cell lines as representative experimental models of the disease
AU - Haley, James
AU - Tomar, Sunil
AU - Pulliam, Nicholas
AU - Xiong, Sen
AU - Perkins, Susan M.
AU - Karpf, Adam R.
AU - Mitra, Sumegha
AU - Nephew, Kenneth P.
AU - Mitra, Anirban K.
N1 - Funding Information:
The research was supported by a Department of Defense Ovarian Cancer Academy Award (AKM), V Foundation Translational Research Award (KPN) and with partial support from Grant Numbers TL1 TR001107 and UL1 TR001108 (A. Shekhar, PI) from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award (NP). SMP was supported by NCI P30 CA082709 (P. Loehrer, PI).
PY - 2016/5/31
Y1 - 2016/5/31
N2 - Genomic analysis of ovarian cancer cell lines has revealed a panel that best represents the most common ovarian cancer subtype, high-grade serous ovarian cancer (HGSOC). However, these HGSOC-like cell lines have not been extensively applied by ovarian cancer researchers to date, and the most commonly used cell lines in the ovarian cancer field do not genetically resemble the major clinical type of the disease. For the HGSOC-like lines to serve as suitable models, they need to be characterized for common functional assays. To achieve that objective, we systematically studied a panel of HGSOC cells CAOV3, COV362, Kuramochi, OVCAR4, OVCAR5, OVCAR8, OVSAHO and SNU119 for migration, invasion, proliferation, clonogenicity, EMT phenotype and cisplatin resistance. They exhibited a range of efficacies and OVCAR5, OVCAR8 and Kuramochi were the most aggressive. SNU119 and OVSAHO cells demonstrated the lowest functional activities. Wide differences in expression of EMT markers were observed between cell lines. SNU119 were the most epithelial and OVCAR8 had the most mesenchymal phenotype. COV362 was the most resistant to cisplatin while CAOV3 was the most sensitive. Taken together, our systematic characterization represents a valuable resource to help guide the application of HGSOC cells by the cancer research community.
AB - Genomic analysis of ovarian cancer cell lines has revealed a panel that best represents the most common ovarian cancer subtype, high-grade serous ovarian cancer (HGSOC). However, these HGSOC-like cell lines have not been extensively applied by ovarian cancer researchers to date, and the most commonly used cell lines in the ovarian cancer field do not genetically resemble the major clinical type of the disease. For the HGSOC-like lines to serve as suitable models, they need to be characterized for common functional assays. To achieve that objective, we systematically studied a panel of HGSOC cells CAOV3, COV362, Kuramochi, OVCAR4, OVCAR5, OVCAR8, OVSAHO and SNU119 for migration, invasion, proliferation, clonogenicity, EMT phenotype and cisplatin resistance. They exhibited a range of efficacies and OVCAR5, OVCAR8 and Kuramochi were the most aggressive. SNU119 and OVSAHO cells demonstrated the lowest functional activities. Wide differences in expression of EMT markers were observed between cell lines. SNU119 were the most epithelial and OVCAR8 had the most mesenchymal phenotype. COV362 was the most resistant to cisplatin while CAOV3 was the most sensitive. Taken together, our systematic characterization represents a valuable resource to help guide the application of HGSOC cells by the cancer research community.
KW - Clonogenicity
KW - Invasion
KW - Migration
KW - Ovarian cancer
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=84973562826&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.9053
DO - 10.18632/oncotarget.9053
M3 - Article
C2 - 27147568
AN - SCOPUS:84973562826
SN - 1949-2553
VL - 7
SP - 32810
EP - 32820
JO - Oncotarget
JF - Oncotarget
IS - 22
ER -