Functional association of Sun1 with nuclear pore complexes

Qian Liu, Nelly Pante, Tom Misteli, Mohamed Elsagga, Melissa Crisp, Didier Hodzic, Brian Burke, Kyle J. Roux

Research output: Contribution to journalArticlepeer-review

157 Scopus citations

Abstract

Sun1 and 2 are A-type lamin-binding proteins that, in association with nesprins, form a link between the inner nuclear membranes (INMs) and outer nuclear membranes of mammalian nuclear envelopes. Both immunofluorescence and immunoelectron microscopy reveal that Sun1 but not Sun2 is intimately associated with nuclear pore complexes (NPCs). Topological analyses indicate that Sun1 is a type II integral protein of the INM. Localization of Sun1 to the INM is defined by at least two discrete regions within its nucleoplasmic domain. However, association with NPCs is dependent on the synergy of both nucleoplasmic and lumenal domains. Cells that are either depleted of Sun1 by RNA interference or that overexpress dominant-negative Sun1 fragments exhibit clustering of NPCs. The implication is that Sun1 represents an important determinant of NPC distribution across the nuclear surface.

Original languageEnglish
Pages (from-to)785-798
Number of pages14
JournalJournal of Cell Biology
Volume178
Issue number5
DOIs
StatePublished - Aug 27 2007

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