Abstract
Objective: To identify the important regulatory elements in the promoter of human CD2 associated protein(CD2AP) by conserved sequence analysis among different species and luciferase functional detection. Methods: The promoter sequences of CD2AP from different species were analyzed by BLAST. Plasmids containing different length of deletion mutations of human CD2AP promoter were constructed. Promoter activities were tested in 3 kinds of cells from different species by luciferase analysis and were tested in HEK-293 cells treated with all-trans-retinoic acid. Results: Homologous sequence comparison in CD2AP promoter area among human, cattle and pig showed that putative specific protein 1 (Sp1) sites and downstream promoter element (DPE) were highly evolutionally conserved. Progressive deletion luciferase analysis of DNA fragments revealed similar promoter activity style among 3 different cell lines from 3 different species, HEK-293, BHK-21 and Vero cells. One basic promoter activity located within 500 bp upstream of ATG. Fragments of further upstream 100 bp or more had drastically 10 times increased promoter activity. Two putative Sp1 sites were in this 100 bp region. All-trans-retinoic acid decreased the luciferase activity of CD2AP promoter. Conclusion: Putative Spl sites and DPE have important functions in the promoter activity of CD2AP.
| Original language | English |
|---|---|
| Pages (from-to) | 805-809 |
| Number of pages | 5 |
| Journal | Chinese Journal of Microbiology and Immunology |
| Volume | 28 |
| Issue number | 9 |
| State | Published - 2008 |
Keywords
- CD2 associated protein
- Glomerularsclerosis
- Nephrotic syndrome
- Promoter