TY - JOUR
T1 - Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function
AU - Derr, Kristy
AU - Zou, Jinyun
AU - Luo, Keren
AU - Song, Min Jae
AU - Sittampalam, G. Sitta
AU - Zhou, Chao
AU - Michael, Sam
AU - Ferrer, Marc
AU - Derr, Paige
N1 - Publisher Copyright:
© Kristy Derr et al. 2019; Published by Mary Ann Liebert, Inc. 2019.
PY - 2019/6
Y1 - 2019/6
N2 - Development of high-throughput, reproducible, three-dimensional (3D) bioprinted skin equivalents (BPSEs) that are morphologically and functionally comparable to native skin tissue is advancing research in skin diseases, and providing a physiologically relevant platform for the development of therapeutics, transplants for regenerative medicine, and testing of skin products like cosmetics. Current protocols for the production of engineered skin grafts are limited in their ability to control 3D geometry of the structure and contraction leading to variability of skin function between constructs. In this study, we describe a method for the biofabrication of skin equivalents (SEs) that are fully bioprinted using an open-market bioprinter, made with commercially available primary cells and natural hydrogels. The unique hydrogel formulation allows for the production of a human-like SE with minimal lateral tissue contraction in a multiwell plate format, thus making them suitable for high-throughput bioprinting in a single print with fast print and relatively short incubation times. The morphology and barrier function of the fully 3D BPSEs are validated by immunohistochemistry staining, optical coherence tomography, and permeation assays. This article describes a method for the biofabrication of skin tissue equivalents in a multiwell plate format. The technique and results overcome shortcomings of previously published engineering methods, and show good architecture and barrier function from well to well; thus it may be used for compound functional testing and for the development of disease tissue models for screening.
AB - Development of high-throughput, reproducible, three-dimensional (3D) bioprinted skin equivalents (BPSEs) that are morphologically and functionally comparable to native skin tissue is advancing research in skin diseases, and providing a physiologically relevant platform for the development of therapeutics, transplants for regenerative medicine, and testing of skin products like cosmetics. Current protocols for the production of engineered skin grafts are limited in their ability to control 3D geometry of the structure and contraction leading to variability of skin function between constructs. In this study, we describe a method for the biofabrication of skin equivalents (SEs) that are fully bioprinted using an open-market bioprinter, made with commercially available primary cells and natural hydrogels. The unique hydrogel formulation allows for the production of a human-like SE with minimal lateral tissue contraction in a multiwell plate format, thus making them suitable for high-throughput bioprinting in a single print with fast print and relatively short incubation times. The morphology and barrier function of the fully 3D BPSEs are validated by immunohistochemistry staining, optical coherence tomography, and permeation assays. This article describes a method for the biofabrication of skin tissue equivalents in a multiwell plate format. The technique and results overcome shortcomings of previously published engineering methods, and show good architecture and barrier function from well to well; thus it may be used for compound functional testing and for the development of disease tissue models for screening.
KW - barrier function
KW - bioprinting
KW - high-throughput screening
KW - keratinocytes
KW - skin
UR - http://www.scopus.com/inward/record.url?scp=85067571552&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2018.0318
DO - 10.1089/ten.tec.2018.0318
M3 - Article
C2 - 31007132
AN - SCOPUS:85067571552
SN - 1937-3384
VL - 25
SP - 334
EP - 343
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
IS - 6
ER -