Fully integrated multiplexed lab-on-a-card assay for enteric pathogens

B. H. Weigl, J. Gerdes, P. Tarr, P. Yager, L. Dillman, R. Peck, S. Ramachandran, M. Lemba, M. Kokorins, M. Nabavi, F. Battrell, D. Hoekstra, E. J. Klein, D. M. Denno

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

18 Scopus citations

Abstract

Under this NIH-fundecl project, we are developing a lab-on-a-card platform to identify enteric bacterial pathogens in patients presenting with acute diarrhea, with special reference to infections that might be encountered in developing countries. Component functions that are integrated on this platform include on-chip immunocapture of live or whole pathogens, multiplexed nucleic acid amplification and on-chip detection, sample processing to support direct use of clinical specimens, and dry reagent storage and handling. All microfluidic functions are contained on the lab card. This new diagnostic test will be able to rapidly identify and differentiate Shigella dysenteriae serotype 1, Shigella toxin-producing Escherichia coli, E. coli 0157, Campylobacter jejuni, and Salmonella and Shigella species. This presentation will report on progress to date on sample and bacteria processing methodologies, identification and validation of capture antibodies and strategy for organism immunocapture, identification and validation of specific polymerase chain reaction (PCR) primer sequences for over 200 clinical isolates of enteric pathogens, and implementation of on-chip nucleic acid extraction for a subset of those pathogens.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - May 3 2006
EventMicrofluidics, BioMEMS, and Medical Microsystems IV - San Jose, CA, United States
Duration: Jan 23 2006Jan 25 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6112
ISSN (Print)0277-786X

Conference

ConferenceMicrofluidics, BioMEMS, and Medical Microsystems IV
Country/TerritoryUnited States
CitySan Jose, CA
Period01/23/0601/25/06

Keywords

  • Enteric disease
  • Lab-on-a-card
  • Lateral flow
  • Microfluidics
  • PCR

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