TY - JOUR
T1 - Free myo-Inositol in Canine Kidneys
T2 - Selective Concentration in the Renal Medulla (41361)
AU - Cohen, Mark A.H.
AU - Hruska, Keith A.
AU - Daughaday, William H.
N1 - Funding Information:
This work was supported in part by NIAMDD Program Project AMO 9976, Diabetes Research and Training Center Grant AM 20579 from the National Institute of Arthritis, Metabolism and Digestive Diseases, Bethesda, Md., and also by American Heart Association Grant in Aid 80-721.
PY - 1982/3
Y1 - 1982/3
N2 - The concentration of free and lipid-bound myo-inositol (MI) was measured in outer and inner cortical zones and in outer and inner medullary zones of the dog kidney. Lipid-bound MI was highest in the outer cortex (0.86 μ mole/g wet wt) and decreased progressively to the inner medulla (0.28 μ mole/g). In contrast free MI was highest in the outer medulla (18.4 ± 2.2 μ mole/g wet wt) as compared to the cortical zones (3.45 ± 0.19 and 5.30 ± 0.33 μ mole/g in outer and inner zones, respectively). The concentration ratio of free MI in outer medullary zone cellular water to plasma water was in excess of 1000. We cannot attribute this high MI concentration to tubular transport because this transport system is present in cortical zone proximal tubules. Water diuresis did not deplete outer medullary MI significantly which suggests that the high medullary MI is independent of the medullary countercurrent mechanism.
AB - The concentration of free and lipid-bound myo-inositol (MI) was measured in outer and inner cortical zones and in outer and inner medullary zones of the dog kidney. Lipid-bound MI was highest in the outer cortex (0.86 μ mole/g wet wt) and decreased progressively to the inner medulla (0.28 μ mole/g). In contrast free MI was highest in the outer medulla (18.4 ± 2.2 μ mole/g wet wt) as compared to the cortical zones (3.45 ± 0.19 and 5.30 ± 0.33 μ mole/g in outer and inner zones, respectively). The concentration ratio of free MI in outer medullary zone cellular water to plasma water was in excess of 1000. We cannot attribute this high MI concentration to tubular transport because this transport system is present in cortical zone proximal tubules. Water diuresis did not deplete outer medullary MI significantly which suggests that the high medullary MI is independent of the medullary countercurrent mechanism.
UR - http://www.scopus.com/inward/record.url?scp=0020471265&partnerID=8YFLogxK
U2 - 10.3181/00379727-169-41361
DO - 10.3181/00379727-169-41361
M3 - Article
C2 - 7063520
AN - SCOPUS:0020471265
SN - 0037-9727
VL - 169
SP - 380
EP - 385
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 3
ER -