Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

Elliot Elson; Thomas M. Jovin

doi:10.1016/0003-2697(69)90023-2

Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

Elliot Elson
, Thomas M. Jovin

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.

Original language	English
Pages (from-to)	193-204
Number of pages	12
Journal	Analytical Biochemistry
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(69)90023-2
State	Published - Feb 1969

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10.1016/0003-2697(69)90023-2

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title = "Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis",

abstract = "Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.",

author = "Elliot Elson and Jovin, \{Thomas M.\}",

note = "Funding Information: ACKNOWLEDGMENTS We are grateful to Dr. L. Stryer for the use of a spectrofluorometer and to Drs. R. L. Baldwin and A. Kornberg for criticism of the manuscript. This work was supported in part by research grants from the U. S. National Institutes of Health (AM 94763) and National Science Foundation (GB 4061). It based in part on the Ph.D. thesis of Elliot Elson, Stanford University, 1966.",

year = "1969",

month = feb,

doi = "10.1016/0003-2697(69)90023-2",

language = "English",

volume = "27",

pages = "193--204",

journal = "Analytical Biochemistry",

issn = "0003-2697",

number = "2",

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T1 - Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

AU - Elson, Elliot

AU - Jovin, Thomas M.

N1 - Funding Information: ACKNOWLEDGMENTS We are grateful to Dr. L. Stryer for the use of a spectrofluorometer and to Drs. R. L. Baldwin and A. Kornberg for criticism of the manuscript. This work was supported in part by research grants from the U. S. National Institutes of Health (AM 94763) and National Science Foundation (GB 4061). It based in part on the Ph.D. thesis of Elliot Elson, Stanford University, 1966.

PY - 1969/2

Y1 - 1969/2

N2 - Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.

AB - Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.

UR - https://www.scopus.com/pages/publications/0014468250

U2 - 10.1016/0003-2697(69)90023-2

DO - 10.1016/0003-2697(69)90023-2

M3 - Article

C2 - 5767178

AN - SCOPUS:0014468250

SN - 0003-2697

VL - 27

SP - 193

EP - 204

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

Abstract

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