Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

Alexander J. Wilson, Qingyu Zhou, Ian Vargas, Rohun Palekar, Ryan Grabau, Hua Pan, Samuel A. Wickline

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-120
Number of pages10
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2118
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Anticoagulation
  • Bivalirudin
  • PPACK
  • Perfluorocarbon nanoparticles
  • Thrombin

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