TY - JOUR
T1 - Fluorometric assay of protein in native human bile
AU - Harvey, P. Robert C.
AU - Upadhya, G. Aravinda
AU - Toth, Julius L.
AU - Strasberg, Steven M.
N1 - Funding Information:
* Supported by the Medical Research Council of Canada. * * Dr. P.R.C. Harvey is an Ontario Ministry of Health Scholar. Correspondence to: Dr. P.R.C. Harvey, 600 University Avenue, Rm. 988, Toronto, Ontario, Canada MSG 1X5.
PY - 1989/8/15
Y1 - 1989/8/15
N2 - Proteins in human native bile samples were determined by a fluorometric assay. Results were compared to biliary proteins quantified by the Lowry and Bradford techniques. The mean protein concentrations in bile as determined by the Lowry, Bradford and fluorometric assays were respectively 7.26 ± 4.52 (SD), 2.9 ± 1.42, and 2.12 ± 1.28 mg/ml (n = 27). Bilirubin was shown to significantly interfere with the Lowry and Bradford assays but not the fluorometric assay. Bile salts remaining in the trichloroacetic acid (TCA) precipitate did not interfere with the fluorometric assay. No cholesterol or phospholipid could be detected in the TCA preparation prior to protein analysis. Proteolytic digestion of proteins in native bile was shown to occur at 37°C and to a lesser extent at 22°C. The fluorometric protein assay is an easy and accurate method to quantitate proteins in native human bile.
AB - Proteins in human native bile samples were determined by a fluorometric assay. Results were compared to biliary proteins quantified by the Lowry and Bradford techniques. The mean protein concentrations in bile as determined by the Lowry, Bradford and fluorometric assays were respectively 7.26 ± 4.52 (SD), 2.9 ± 1.42, and 2.12 ± 1.28 mg/ml (n = 27). Bilirubin was shown to significantly interfere with the Lowry and Bradford assays but not the fluorometric assay. Bile salts remaining in the trichloroacetic acid (TCA) precipitate did not interfere with the fluorometric assay. No cholesterol or phospholipid could be detected in the TCA preparation prior to protein analysis. Proteolytic digestion of proteins in native bile was shown to occur at 37°C and to a lesser extent at 22°C. The fluorometric protein assay is an easy and accurate method to quantitate proteins in native human bile.
KW - Bile
KW - Biliary protein
KW - Fluorescence
UR - http://www.scopus.com/inward/record.url?scp=0024359716&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(89)90330-6
DO - 10.1016/0009-8981(89)90330-6
M3 - Article
C2 - 2676244
AN - SCOPUS:0024359716
SN - 0009-8981
VL - 183
SP - 147
EP - 154
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -