Fluorometric assay of protein in native human bile

P. Robert C. Harvey, G. Aravinda Upadhya, Julius L. Toth, Steven M. Strasberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Proteins in human native bile samples were determined by a fluorometric assay. Results were compared to biliary proteins quantified by the Lowry and Bradford techniques. The mean protein concentrations in bile as determined by the Lowry, Bradford and fluorometric assays were respectively 7.26 ± 4.52 (SD), 2.9 ± 1.42, and 2.12 ± 1.28 mg/ml (n = 27). Bilirubin was shown to significantly interfere with the Lowry and Bradford assays but not the fluorometric assay. Bile salts remaining in the trichloroacetic acid (TCA) precipitate did not interfere with the fluorometric assay. No cholesterol or phospholipid could be detected in the TCA preparation prior to protein analysis. Proteolytic digestion of proteins in native bile was shown to occur at 37°C and to a lesser extent at 22°C. The fluorometric protein assay is an easy and accurate method to quantitate proteins in native human bile.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalClinica Chimica Acta
Volume183
Issue number2
DOIs
StatePublished - Aug 15 1989
Externally publishedYes

Keywords

  • Bile
  • Biliary protein
  • Fluorescence

Fingerprint Dive into the research topics of 'Fluorometric assay of protein in native human bile'. Together they form a unique fingerprint.

  • Cite this