Fluorescent labeling of yeast.

J. J. Baggett; J. D. Shaw; C. J. Sciambi; H. A. Watson; B. Wendland

Fluorescent labeling of yeast.

J. J. Baggett
, J. D. Shaw
, C. J. Sciambi
, H. A. Watson
, B. Wendland

Washington University in St. Louis

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

Original language	English
Pages (from-to)	Unit 4.13
Journal	Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
Volume	Chapter 4
State	Published - Nov 2003

Cite this

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title = "Fluorescent labeling of yeast.",

abstract = "This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.",

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AU - Baggett, J. J.

AU - Shaw, J. D.

AU - Sciambi, C. J.

AU - Watson, H. A.

AU - Wendland, B.

PY - 2003/11

Y1 - 2003/11

N2 - This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

AB - This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

UR - https://www.scopus.com/pages/publications/40349098687

M3 - Article

C2 - 18228435

AN - SCOPUS:40349098687

SN - 1934-2500

VL - Chapter 4

SP - Unit 4.13

JO - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

JF - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

ER -

Fluorescent labeling of yeast.

Abstract

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