Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography

John Turk, Stephen J. Weiss, J. E. Davis, Philip Needleman

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method.

Original languageEnglish
Pages (from-to)291-309
Number of pages19
JournalProstaglandins
Volume16
Issue number2
DOIs
StatePublished - Aug 1978

Fingerprint

Dive into the research topics of 'Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography'. Together they form a unique fingerprint.

Cite this