Abstract
Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method.
Original language | English |
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Pages (from-to) | 291-309 |
Number of pages | 19 |
Journal | Prostaglandins |
Volume | 16 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1978 |