Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography

John Turk; Stephen J. Weiss; J. E. Davis; Philip Needleman

doi:10.1016/0090-6980(78)90031-X

Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography

John Turk, Stephen J. Weiss, J. E. Davis, Philip Needleman

Division of Endocrinology, Metabolism & Lipid Research

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Fluorescent esters of the prostablandins D₂, E₂, F₂α, and 6-keto-F₁α and of thromboxane B₂ have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE₂ can be detected after derivatization and HPLC analysis. Identification of thromboxane B₂ produced by human platelets and of 6-keto-PG F₁α produced by bovine aortic microsomes has been achieved with this method.

Original language	English
Pages (from-to)	291-309
Number of pages	19
Journal	Prostaglandins
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/0090-6980(78)90031-X
State	Published - Aug 1978

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title = "Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography",

abstract = "Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method.",

author = "John Turk and Weiss, {Stephen J.} and Davis, {J. E.} and Philip Needleman",

note = "Funding Information: This study was supported by grants from the National Institutes of Health (SCOR-HL-17646 and HL-20787) to Philip Needleman. We are indebted to Angela Wyche for invaluable technical assistance.",

year = "1978",

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doi = "10.1016/0090-6980(78)90031-X",

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T1 - Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography

AU - Turk, John

AU - Weiss, Stephen J.

AU - Davis, J. E.

AU - Needleman, Philip

N1 - Funding Information: This study was supported by grants from the National Institutes of Health (SCOR-HL-17646 and HL-20787) to Philip Needleman. We are indebted to Angela Wyche for invaluable technical assistance.

PY - 1978/8

Y1 - 1978/8

N2 - Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method.

AB - Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method.

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VL - 16

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JO - Prostaglandins

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ER -

Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography

Abstract

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