Fluorescence Correlation Spectroscopy and Phase Separation

Juan Jeremías Incicco, Debjit Roy, Melissa D. Stuchell-Brereton, Andrea Soranno

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages38
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Biomolecular condensates
  • Fluorescence Correlation Spectroscopy
  • Intrinsically disordered proteins
  • Phase separation


Dive into the research topics of 'Fluorescence Correlation Spectroscopy and Phase Separation'. Together they form a unique fingerprint.

Cite this