@inbook{3bdb5d81c4bd4d2a99f325328542e6cd,
title = "Fluorescence Correlation Spectroscopy and Phase Separation",
abstract = "A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.",
keywords = "Biomolecular condensates, Fluorescence Correlation Spectroscopy, Intrinsically disordered proteins, Phase separation",
author = "Incicco, {Juan Jerem{\'i}as} and Debjit Roy and Stuchell-Brereton, {Melissa D.} and Andrea Soranno",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2663-4_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "161--198",
booktitle = "Methods in Molecular Biology",
}