Fluorescence Correlation Spectroscopy: A Tool for Measuring Dynamic and Equilibrium Properties of Molecules in Cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescence correlation spectroscopy (FCS) measures dynamic processes of fluorescent molecules including diffusion, convection, and chemical reaction and equilibrium properties such as molecular concentrations and aggregation states. The measurements are carried out on low concentrations of fluorescent molecules (nanomolar), in tiny volumes (femtoliter), and over a wide time range (microseconds to seconds). FCS measures correlations of fluorescence intensity from individual fluorescent molecules and therefore inherently is a single molecule technique. FCS is well suited to measurements of molecular processes in cells and has been used to study diffusion, molecular associations, and assembly of cellular structures. A complementary, closely related approach, fluorescence photobleaching recovery (FRAP) is suited for high concentrations of fluorescent molecules and slower times (100 milliseconds to seconds).

Original languageEnglish
Title of host publicationOrganizational Cell Biology
PublisherElsevier Inc.
Pages99-106
Number of pages8
Volume2
ISBN (Electronic)9780123944474
ISBN (Print)9780123947963
DOIs
StatePublished - Jan 1 2016

Keywords

  • Aggregation
  • Autocorrelation function
  • Chemical rate constants
  • Chemical reaction kinetics
  • Diffusion
  • Diffusion coefficient
  • Fluorescence correlation spectroscopy
  • Fluorescence fluctuations
  • Photobleaching recovery
  • Photon count histogram
  • Polymerization

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