Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses

E. G.M. Berkhoff; E. de Wit; M. M. Geelhoed-Mieras; A. C.M. Boon; J. Symons; R. A.M. Fouchier; A. D.M.E. Osterhaus; G. F. Rimmelzwaan

doi:10.1016/j.vaccine.2006.05.051

Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses

E. G.M. Berkhoff, E. de Wit, M. M. Geelhoed-Mieras, A. C.M. Boon, J. Symons, R. A.M. Fouchier, A. D.M.E. Osterhaus, G. F. Rimmelzwaan

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37 Scopus citations

Abstract

The use of cytotoxic T lymphocyte (CTL)-inducing vaccines could afford both homo- and heterosubtypic immunity. However, amino acid variation in CTL epitopes associated with escape from CTL-mediated immunity might undermine the use of these vaccines. To assess the impact of amino acid substitutions in highly conserved epitopes on viral fitness and recognition by specific CTL, we performed a mutational analysis of various CTL epitopes. Our findings indicated that fitness costs limited variation in functionally constrained epitopes, especially at anchor residues.

Original language	English
Pages (from-to)	6594-6596
Number of pages	3
Journal	Vaccine
Volume	24
Issue number	44-46
DOIs	https://doi.org/10.1016/j.vaccine.2006.05.051
State	Published - Nov 10 2006

Keywords

Anchor residues
CTL epitopes
Cytotoxic T lymphocytes
Escape
Influenza A virus

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10.1016/j.vaccine.2006.05.051

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title = "Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses",

abstract = "The use of cytotoxic T lymphocyte (CTL)-inducing vaccines could afford both homo- and heterosubtypic immunity. However, amino acid variation in CTL epitopes associated with escape from CTL-mediated immunity might undermine the use of these vaccines. To assess the impact of amino acid substitutions in highly conserved epitopes on viral fitness and recognition by specific CTL, we performed a mutational analysis of various CTL epitopes. Our findings indicated that fitness costs limited variation in functionally constrained epitopes, especially at anchor residues.",

keywords = "Anchor residues, CTL epitopes, Cytotoxic T lymphocytes, Escape, Influenza A virus",

author = "Berkhoff, {E. G.M.} and {de Wit}, E. and Geelhoed-Mieras, {M. M.} and Boon, {A. C.M.} and J. Symons and Fouchier, {R. A.M.} and Osterhaus, {A. D.M.E.} and Rimmelzwaan, {G. F.}",

note = "Funding Information: This work was supported by European Union grant QLRT-2001-01034 (Novaflu). The authors thank T.M. Bestebroer and M.I.J. Spronken for excellent technical assistance.",

year = "2006",

month = nov,

day = "10",

doi = "10.1016/j.vaccine.2006.05.051",

language = "English",

volume = "24",

pages = "6594--6596",

journal = "Vaccine",

issn = "0264-410X",

number = "44-46",

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TY - JOUR

T1 - Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses

AU - Berkhoff, E. G.M.

AU - de Wit, E.

AU - Geelhoed-Mieras, M. M.

AU - Boon, A. C.M.

AU - Symons, J.

AU - Fouchier, R. A.M.

AU - Osterhaus, A. D.M.E.

AU - Rimmelzwaan, G. F.

N1 - Funding Information: This work was supported by European Union grant QLRT-2001-01034 (Novaflu). The authors thank T.M. Bestebroer and M.I.J. Spronken for excellent technical assistance.

PY - 2006/11/10

Y1 - 2006/11/10

N2 - The use of cytotoxic T lymphocyte (CTL)-inducing vaccines could afford both homo- and heterosubtypic immunity. However, amino acid variation in CTL epitopes associated with escape from CTL-mediated immunity might undermine the use of these vaccines. To assess the impact of amino acid substitutions in highly conserved epitopes on viral fitness and recognition by specific CTL, we performed a mutational analysis of various CTL epitopes. Our findings indicated that fitness costs limited variation in functionally constrained epitopes, especially at anchor residues.

AB - The use of cytotoxic T lymphocyte (CTL)-inducing vaccines could afford both homo- and heterosubtypic immunity. However, amino acid variation in CTL epitopes associated with escape from CTL-mediated immunity might undermine the use of these vaccines. To assess the impact of amino acid substitutions in highly conserved epitopes on viral fitness and recognition by specific CTL, we performed a mutational analysis of various CTL epitopes. Our findings indicated that fitness costs limited variation in functionally constrained epitopes, especially at anchor residues.

KW - Anchor residues

KW - CTL epitopes

KW - Cytotoxic T lymphocytes

KW - Escape

KW - Influenza A virus

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U2 - 10.1016/j.vaccine.2006.05.051

DO - 10.1016/j.vaccine.2006.05.051

M3 - Article

C2 - 16837112

AN - SCOPUS:33846193734

SN - 0264-410X

VL - 24

SP - 6594

EP - 6596

JO - Vaccine

JF - Vaccine

IS - 44-46

ER -

Fitness costs limit escape from cytotoxic T lymphocytes by influenza A viruses

Abstract

Keywords

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