FIP200 regulates targeting of Atg16L1 to the isolation membrane

Taki Nishimura, Takeshi Kaizuka, Ken Cadwell, Mayurbhai H. Sahani, Tatsuya Saitoh, Shizuo Akira, Herbert W. Virgin, Noboru Mizushima

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Autophagosome formation is a dynamic process that is strictly controlled by autophagy-related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14-and phosphatidylinositol 3-kinase-independent manner. Atg16L1 deletion mutants, which lack the FIP200-interacting domain, are defective in proper membrane targeting. Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1.

Original languageEnglish
Pages (from-to)284-291
Number of pages8
JournalEMBO Reports
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2013

Keywords

  • Atg16L1
  • FIP200
  • ULK1
  • autophagy

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    Nishimura, T., Kaizuka, T., Cadwell, K., Sahani, M. H., Saitoh, T., Akira, S., Virgin, H. W., & Mizushima, N. (2013). FIP200 regulates targeting of Atg16L1 to the isolation membrane. EMBO Reports, 14(3), 284-291. https://doi.org/10.1038/embor.2013.6