Fingerprinting microbial genomes using the RAPD or AP-PCR method

D. E. Berg, N. S. Akopyants, D. Kersulyte

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

The random amplified polymorphic DNA (RAPD) or arbitrarily primed polymerase chain reaction (AP-PCR) DNA fingerprinting technique provides one of the most sensitive and efficient of current methods for distinguishing different strains of a species. It uses single oligonucleotides of arbitrarily chosen sequence with no known homology to the target genome as primers and yields arrays of DNA fragments that are strain-specific and generally reproducible. Some of the conditions that have been found to be optimal for RAPD tests of representative microbes are described along with possible interpretations of test results.

Original languageEnglish
Pages (from-to)13-24
Number of pages12
JournalMethods in Molecular and Cellular Biology
Volume5
Issue number1
StatePublished - Jan 1 1994

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