TY - JOUR
T1 - Filter matrix estimation in automated DNA sequencing
AU - Huang, Weian
AU - Fuhrmann, Daniel R.
AU - Politte, David G.
AU - Thomas, Lewis J.
AU - States, David J.
N1 - Funding Information:
Manuscript received April 3, 1996; revised November 26, 1997. This work was supported in part by the National Institutes of Health under Grants RR01380, HG0031, and HG01391. Asterisk indicates corresponding author. W. Huang was with the Institute for Biomedical Computing, Washington University, St. Louis, MO 63110 USA. He is now with the Mayo Clinic, Rochester, MN 55905 USA. *D. R. Fuhrmann is with the Electronic Systems and Signals Research Laboratory, Department of Electrical Engineering, Washington University, St. Louis, MO 63130 USA (e-mail: [email protected]). D. G. Politte, L. J. Thomas, Jr, and D. J. States are with the Institute for Biomedical Computing, Washington University, St. Louis, MO 63110 USA. Publisher Item Identifier S 0018-9294(98)01793-5.
PY - 1998
Y1 - 1998
N2 - In four-color fluourescence-based automated DNA sequencing, a 4 x 4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. In this paper, we present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.
AB - In four-color fluourescence-based automated DNA sequencing, a 4 x 4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. In this paper, we present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.
KW - Electro-phoresis
KW - Filter matrix
KW - Fluorescencebased DNA sequencing
UR - http://www.scopus.com/inward/record.url?scp=0032053975&partnerID=8YFLogxK
U2 - 10.1109/10.664198
DO - 10.1109/10.664198
M3 - Article
C2 - 9556959
AN - SCOPUS:0032053975
SN - 0018-9294
VL - 45
SP - 422
EP - 428
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
IS - 4
ER -