@article{ad0f153c7f0d4702a6614ccc258a4e03,
title = "Filter matrix estimation in automated DNA sequencing",
abstract = "In four-color fluourescence-based automated DNA sequencing, a 4 x 4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. In this paper, we present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.",
keywords = "Electro-phoresis, Filter matrix, Fluorescencebased DNA sequencing",
author = "Weian Huang and Fuhrmann, {Daniel R.} and Politte, {David G.} and Thomas, {Lewis J.} and States, {David J.}",
note = "Funding Information: Manuscript received April 3, 1996; revised November 26, 1997. This work was supported in part by the National Institutes of Health under Grants RR01380, HG0031, and HG01391. Asterisk indicates corresponding author. W. Huang was with the Institute for Biomedical Computing, Washington University, St. Louis, MO 63110 USA. He is now with the Mayo Clinic, Rochester, MN 55905 USA. *D. R. Fuhrmann is with the Electronic Systems and Signals Research Laboratory, Department of Electrical Engineering, Washington University, St. Louis, MO 63130 USA (e-mail: daff@saturn.wustl.edu). D. G. Politte, L. J. Thomas, Jr, and D. J. States are with the Institute for Biomedical Computing, Washington University, St. Louis, MO 63110 USA. Publisher Item Identifier S 0018-9294(98)01793-5.",
year = "1998",
doi = "10.1109/10.664198",
language = "English",
volume = "45",
pages = "422--428",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
number = "4",
}