TY - JOUR
T1 - Filarial antigenemia and Loa loa night blood microfilaremia in an area without bancroftian filariasis in the democratic republic of Congo
AU - Bakajika, Didier K.
AU - Nigo, Maurice M.
AU - Lotsima, Jean Pierre
AU - Masikini, Germain A.
AU - Fischer, Kerstin
AU - Lloyd, Melanie M.
AU - Weil, Gary J.
AU - Fischer, Peter U.
N1 - Publisher Copyright:
Copyright © 2014 by The American Society of Tropical Medicine and Hygiene.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Implementation of mass drug administration for lymphatic filariasis (LF) has been delayed in central Africa because of incomplete mapping and coendemic loiasis. We mapped two regions in eastern Democratic Republic of Congo that were suspected to have LF. Night blood samples were collected from 2,724 subjects in 30 villages. Filarial antigenemia rates by card test exceeded 1% in 28 villages (range = 0-14%). Prevalence rates for large sheathed microfilariae (Mf) ranged from 4% to 40%; Mansonella perstans rates ranged from 22% to 98%. Large Mf were exclusively Loa loa by microscopy, and only 1 of 337 samples tested by quantitative polymerase chain reaction (qPCR) was positive for Wuchereria bancrofti DNA. Filarial antigen positivity was strongly associated with high L. loa Mf counts. Periodicity studies revealed atypical patterns, with no significant diurnal periodicity in some individuals. Thus, methods routinely used for LF mapping may not be reliable in areas in central Africa that are highly endemic for loiasis.
AB - Implementation of mass drug administration for lymphatic filariasis (LF) has been delayed in central Africa because of incomplete mapping and coendemic loiasis. We mapped two regions in eastern Democratic Republic of Congo that were suspected to have LF. Night blood samples were collected from 2,724 subjects in 30 villages. Filarial antigenemia rates by card test exceeded 1% in 28 villages (range = 0-14%). Prevalence rates for large sheathed microfilariae (Mf) ranged from 4% to 40%; Mansonella perstans rates ranged from 22% to 98%. Large Mf were exclusively Loa loa by microscopy, and only 1 of 337 samples tested by quantitative polymerase chain reaction (qPCR) was positive for Wuchereria bancrofti DNA. Filarial antigen positivity was strongly associated with high L. loa Mf counts. Periodicity studies revealed atypical patterns, with no significant diurnal periodicity in some individuals. Thus, methods routinely used for LF mapping may not be reliable in areas in central Africa that are highly endemic for loiasis.
UR - http://www.scopus.com/inward/record.url?scp=84918780772&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.14-0358
DO - 10.4269/ajtmh.14-0358
M3 - Article
C2 - 25223938
AN - SCOPUS:84918780772
VL - 91
SP - 1142
EP - 1148
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 6
ER -