Fibulin-2 is dispensable for mouse development and elastic fiber formation

Francois Xavier Sicot, Takeshi Tsuda, Dessislava Markova, John F. Klement, Machiko Arita, Rui Zhu Zhang, Te Cheng Pan, Robert P. Mecham, David E. Birk, Mon Li Chu

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Fibulin-2 is an extracellular matrix protein belonging to the five-member fibulin family, of which two members have been shown to play essential roles in elastic fiber formation during development. Fibulin-2 interacts with two major constituents of elastic fibers, tropoelastin and fibrillin-1, in vitro and localizes to elastic fibers in many tissues in vivo. The protein is prominently expressed during morphogenesis of the heart and aortic arch vessels and at early stages of cartilage development. To examine its role in vivo, we generated mice that do not express the fibulin-2 gene (Fbln2) through homologous recombination of embryonic stem cells. Unexpectedly, the fibulin-2-null mice were viable and fertile and did not display gross and anatomical abnormalities. Histological and ultrastructural analyses revealed that elastic fibers assembled normally in the absence of fibulin-2. No compensatory up-regulation of mRNAs for other fibulin members was detected in the aorta and skin tissue. However, in the fibulin-2 null aortae, fibulin-1 immunostaining was increased in the inner elastic lamina, where fibulin-2 preferentially localizes. The results demonstrate that fibulin-2 is not required for mouse development and elastic fiber formation and suggest possible functional redundancy between fibulin-1 and fibulin-2.

Original languageEnglish
Pages (from-to)1061-1067
Number of pages7
JournalMolecular and cellular biology
Volume28
Issue number3
DOIs
StatePublished - Feb 2008

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