Fibrillin-1 and -2 contain heparin-binding sites important for matrix deposition and that support cell attachment

Timothy M. Ritty, Thomas J. Broekelmann, Claudio C. Werneck, Robert P. Mecham

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Fibrillin-1 and -2 are large modular extracellular matrix glyco-proteins found in many vertebrate organ systems and are known to be key components of the elastic fibre. In the present study, we identify a new heparin-binding region in fibrillin-2 between exons 18 and 24. Additionally, we have narrowed the location of heparin-binding activity previously identified in fibrillin-1 to the last 17 residues of the mature proteolytically processed protein. This domain demonstrated higher activity as a multimer than as a monomer. The fibrillin-1 C-terminal site supported cell attachment in each of nine cell types tested. Attachment was shown to be mediated by cell-surface heparan sulphate proteoglycans. Fibrillin-1 has been shown previously to have heparin-binding activity that is important for matrix deposition of the molecule by fibroblasts. This function in deposition was confirmed in two additional fibrillin-producing cell types (osteosarcoma and epithelial cells) for the deposition of both fibrillin-1 and -2 into the extracellular matrix.

Original languageEnglish
Pages (from-to)425-432
Number of pages8
JournalBiochemical Journal
Volume375
Issue number2
DOIs
StatePublished - Oct 15 2003

Keywords

  • Extracellular matrix
  • Fibrillin
  • Heparin/heparan sulphate binding

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