TY - JOUR
T1 - Fiber photometry in striatum reflects primarily nonsomatic changes in calcium
AU - Legaria, Alex A.
AU - Matikainen-Ankney, Bridget A.
AU - Yang, Ben
AU - Ahanonu, Biafra
AU - Licholai, Julia A.
AU - Parker, Jones G.
AU - Kravitz, Alexxai V.
N1 - Funding Information:
We thank the HHMI GENIE project for GCaMP reagents and M. Creed for critical reading of the manuscript. Research was supported by the American Heart Association Pre-Doctoral Fellowship (A.A.L), NIDDK DK126355 (B.A.M.-A.), Howard Hughes Medical Institute Hanna H. Gray Fellowship (B.A.), NINDS R35 Diversity Research Supplement Funding, 3R35NS097306-04S1 (B.A.), NINDS R01NS122840 (J.G.P.), Washington University Diabetes Research Center (DK020579, A.V.K.), Nutrition Obesity Research Center (DK056341, A.V.K.), McDonnell Centers for Systems and Cellular Neuroscience (A.V.K.).
Funding Information:
We thank the HHMI GENIE project for GCaMP reagents and M. Creed for critical reading of the manuscript. Research was supported by the American Heart Association Pre-Doctoral Fellowship (A.A.L), NIDDK DK126355 (B.A.M.-A.), Howard Hughes Medical Institute Hanna H. Gray Fellowship (B.A.), NINDS R35 Diversity Research Supplement Funding, 3R35NS097306-04S1 (B.A.), NINDS R01NS122840 (J.G.P.), Washington University Diabetes Research Center (DK020579, A.V.K.), Nutrition Obesity Research Center (DK056341, A.V.K.), McDonnell Centers for Systems and Cellular Neuroscience (A.V.K.).
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2022/9
Y1 - 2022/9
N2 - Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, ‘spiking’) or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.
AB - Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, ‘spiking’) or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.
UR - http://www.scopus.com/inward/record.url?scp=85137064029&partnerID=8YFLogxK
U2 - 10.1038/s41593-022-01152-z
DO - 10.1038/s41593-022-01152-z
M3 - Article
C2 - 36042311
AN - SCOPUS:85137064029
SN - 1097-6256
VL - 25
SP - 1124
EP - 1128
JO - Nature neuroscience
JF - Nature neuroscience
IS - 9
ER -