FGF9 regulates early hypertrophic chondrocyte differentiation and skeletal vascularization in the developing stylopod

Irene H. Hung, Kai Yu, Kory J. Lavine, David M. Ornitz

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Gain-of-function mutations in fibroblast growth factor (FGF) receptors result in chondrodysplasia and craniosynostosis syndromes, highlighting the critical role for FGF signaling in skeletal development. Although the FGFRs involved in skeletal development have been well characterized, only a single FGF ligand, FGF18, has been identified that regulates skeletal development during embryogenesis. Here we identify Fgf9 as a second FGF ligand that is critical for skeletal development. We show that Fgf9 is expressed in the proximity of developing skeletal elements and that Fgf9-deficient mice exhibit rhizomelia (a disproportionate shortening of proximal skeletal elements), which is a prominent feature of patients with FGFR3-induced chondrodysplasia syndromes. Although Fgf9 is expressed in the apical ectodermal ridge in the limb bud, we demonstrate that the Fgf9-/- limb phenotype results from loss of FGF9 functions after formation of the mesenchymal condensation. In developing stylopod elements, FGF9 promotes chondrocyte hypertrophy at early stages and regulates vascularization of the growth plate and osteogenesis at later stages of skeletal development.

Original languageEnglish
Pages (from-to)300-313
Number of pages14
JournalDevelopmental Biology
Volume307
Issue number2
DOIs
StatePublished - Jul 15 2007

Keywords

  • Chondrocyte
  • Fibroblast growth factor 9 (FGF9)
  • Growth plate
  • Osteoblast
  • Perichondrium
  • Periosteum
  • Skeletal development
  • Vascular development

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