Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function

Marianela Masin, Daniel Kerschensteiner, Kerstin Dümke, Maria E. Rubio, Florentina Soto

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Ionotropic receptors in the neuronal plasma membrane are organized in macromolecular complexes, which assure their proper localization and regulate signal transduction. P2X receptors, the ionotropic receptors activated by extracellular ATP, have been shown to influence synaptic transmission. Using a yeast two-hybrid approach with the P2X2 subunit C-terminal domain as bait we isolated the β-amyloid precursor protein-binding proteins Fe65 and Fe65-like 1 as the first identified proteins interacting with neuronal P2X receptors. We confirmed the direct interaction of Fe65 and the P2X2 C-terminal domain by glutathione S-transferase pull-down experiments. No interaction was observed between Fe65 and the naturally occurring P2X 2 splice variant P2X2(b), indicating that alternative splicing can regulate the receptor complex assembly. We generated two antibodies to Fe65 to determine its subcellular localization using postembedding immunogold labeling electron microscopy. We found labeling for Fe65 at the pre- and postsynaptic specialization of CA1 hippocampal pyramidal cell/Schaffer collateral synapses. By double immunogold labeling, we determined that Fe65 colocalizes with P2X2 subunits at the postsynaptic specialization of excitatory synapses. Moreover, P2X2 and Fe65 could be coimmunoprecipitated from brain membrane extracts, demonstrating that the interaction occurs in vivo. The assembly with Fe65 regulates the functional properties of P2X2 receptors. Thus, the time- and activation-dependent change in ionic selectivity of P2X2 receptors was inhibited by coexpression of Fe65, suggesting a novel role for Fe65 in regulating P2X receptor function and ATP-mediated synaptic transmission.

Original languageEnglish
Pages (from-to)4100-4108
Number of pages9
JournalJournal of Biological Chemistry
Issue number7
StatePublished - Feb 17 2006


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