TY - JOUR
T1 - Fatty acid remodeling
T2 - A novel reaction sequence in the biosynthesis of trypanosome glycosyl phosphatidylinositol membrane anchors
AU - Masterson, Wayne J.
AU - Raper, Jayne
AU - Doering, Tamara L.
AU - Hart, Gerald W.
AU - Englund, Paul T.
N1 - Funding Information:
We thank Larry Buxbaum, Kojo Mensa-Wilmot, Melissa Pessin. and Dan Raben for invaluable help and discussion. We appreciate many contributions from Viiu Klein and Shirley Skiles and the provision of unpublished manuscripts by Anant Menon. Financial support was from the National Institutes of Health (A121334) and the MacArthur Foundation; T L. D. was supported by NIH Medical Scientist Training Grant 5T32GMO7309.
PY - 1990/7/13
Y1 - 1990/7/13
N2 - The trypanosome variant surface glycoprotein (VSG) is anchored to the plasma membrane via a glycosyl phosphatidylinositol (GPI). The GPI is synthesized as a precursor, glycolipid A, that is subsequently linked to the VSG polypeptide. The VSG anchor is unusual, compared with anchors in other cell types, in that its fatty acid moieties are exclusively myristic acid. To investigate the mechanism for myristate specificity we used a cell-free system for GPI biosynthesis. One product of this system, glycolipid A′, is indistinguishable from glycolipid A except that its fatty acids are more hydrophobic than myristate. Glycolipid A′ is converted to glycolipid A through highly specific fatty acid remodeling reactions involving deacylation and subsequent reacylation with myristate. Therefore, myristoylation occurs in the final phase of trypanosome GPI biosynthesis.
AB - The trypanosome variant surface glycoprotein (VSG) is anchored to the plasma membrane via a glycosyl phosphatidylinositol (GPI). The GPI is synthesized as a precursor, glycolipid A, that is subsequently linked to the VSG polypeptide. The VSG anchor is unusual, compared with anchors in other cell types, in that its fatty acid moieties are exclusively myristic acid. To investigate the mechanism for myristate specificity we used a cell-free system for GPI biosynthesis. One product of this system, glycolipid A′, is indistinguishable from glycolipid A except that its fatty acids are more hydrophobic than myristate. Glycolipid A′ is converted to glycolipid A through highly specific fatty acid remodeling reactions involving deacylation and subsequent reacylation with myristate. Therefore, myristoylation occurs in the final phase of trypanosome GPI biosynthesis.
UR - https://www.scopus.com/pages/publications/0025350498
U2 - 10.1016/0092-8674(90)90241-6
DO - 10.1016/0092-8674(90)90241-6
M3 - Article
C2 - 1694728
AN - SCOPUS:0025350498
SN - 0092-8674
VL - 62
SP - 73
EP - 80
JO - Cell
JF - Cell
IS - 1
ER -