Fat feeding stimulates only one of the two mRNAs encoding rat intestinal membranous and secreted alkaline phosphatase

Shakuntla Seetharam, Catherine Ovitt, Arnold W. Strauss, Deborah Rubin, David H. Alpers

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have identified two mRNAs in rat intestinal mucosa by Northern blot analysis, using cloned cDNAs encoding human placental alkaline phosphatase (PLAP). Probes from both the NH2- and COOH-terminal ends of the human PLAP coding region identified, in rat intestine (especially duodenum), an mRNA of nearly identical size (3 kb) to that found in human placenta. A smaller mRNA (2.7 kb), detected only with the COOH-terminal probe, was more prevalent in jejunum. Following feeding of triacylglycerols, the prevalance of the 2.7 kb mRNA increased over 2-fold. The tissue distribution and response of the 2.7 kb mRNA to fat feeding corresponds exactly with the known behavior of the secreted alkaline phosphatase.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number1
DOIs
StatePublished - May 29 1987

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