Fast Protein Footprinting by X-ray Mediated Radical Trifluoromethylation

Ming Cheng; Awuri Asuru; Janna Kiselar; George Mathai; Mark R. Chance; Michael L. Gross

doi:10.1021/jasms.0c00085

Fast Protein Footprinting by X-ray Mediated Radical Trifluoromethylation

Ming Cheng, Awuri Asuru, Janna Kiselar, George Mathai, Mark R. Chance, Michael L. Gross

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Synchrotron radiolysis generates hydroxyl radicals (·OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (·CF3). The radical is produced by ·OH displacement of ·CF3 from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on a millisecond or shorter time scale. The ·CF3 is comparably reactive to ·OH and produces footprinting information that complements that of ·OH alone. This reagent in combination with ·OH should enable novel chemistry for protein footprinting on the synchrotron platform.

Original language	English
Pages (from-to)	1019-1024
Number of pages	6
Journal	Journal of the American Society for Mass Spectrometry
Volume	31
Issue number	5
DOIs	https://doi.org/10.1021/jasms.0c00085
State	Published - May 6 2020

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abstract = "Synchrotron radiolysis generates hydroxyl radicals (·OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (·CF3). The radical is produced by ·OH displacement of ·CF3 from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on a millisecond or shorter time scale. The ·CF3 is comparably reactive to ·OH and produces footprinting information that complements that of ·OH alone. This reagent in combination with ·OH should enable novel chemistry for protein footprinting on the synchrotron platform.",

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AU - Chance, Mark R.

AU - Gross, Michael L.

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AB - Synchrotron radiolysis generates hydroxyl radicals (·OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (·CF3). The radical is produced by ·OH displacement of ·CF3 from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on a millisecond or shorter time scale. The ·CF3 is comparably reactive to ·OH and produces footprinting information that complements that of ·OH alone. This reagent in combination with ·OH should enable novel chemistry for protein footprinting on the synchrotron platform.

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Fast Protein Footprinting by X-ray Mediated Radical Trifluoromethylation

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