Fast photochemical oxidation of proteins for epitope mapping

Lisa M. Jones, Justin B. Sperry, James A. Carroll, Michael L. Gross

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


The growing use of monoclonal antibodies as therapeutics underscores the importance of epitope mapping as an essential step in characterizing antibody-antigen complexes. The use of protein footprinting coupled with mass spectrometry, which is emerging as a tool in structural biology, offers opportunities to map antibody-binding regions of antigens. We report here the use of footprinting via fast photochemical oxidation of proteins (FPOP) with OH radicals to characterize the epitope of the serine protease thrombin. The data correlate well with previously published results that determined the epitope of thrombin. This study marks the first time oxidative labeling has been used for epitope mapping.

Original languageEnglish
Pages (from-to)7657-7661
Number of pages5
JournalAnalytical Chemistry
Issue number20
StatePublished - Oct 15 2011


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