Fast atom bombardment combined with tandem mass spectrometry for the determination of nucleosides

Frank W. Crow; Kenneth B. Tomer; Michael L. Gross; James A. McCloskey; Donald E. Bergstrom

doi:10.1016/0003-2697(84)90412-3

Fast atom bombardment combined with tandem mass spectrometry for the determination of nucleosides

Frank W. Crow
, Kenneth B. Tomer
, Michael L. Gross
, James A. McCloskey
, Donald E. Bergstrom

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

The positive and negative ion fast atom bombardment (FAB) mass spectra and fast atom bombardment collisionally activated decomposition (CAD) spectra of a series of nucleosides and two dinucleotides are reported. The nucleosides studied are substituted forms of guanosine, adenosine, nebularine, tubercidin, uridine, and related pyrimidines. The FAB and CAD data both contain similar information. The CAD spectra are found to provide some structural information not found in the FAB mass spectra. Tandem mass spectrometry also allows emphasis to be put on weak fragments which are either not observed in the FAB mass spectrum or are lost in the matrix ion signals.

Original language	English
Pages (from-to)	243-262
Number of pages	20
Journal	Analytical Biochemistry
Volume	139
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90412-3
State	Published - May 15 1984

Keywords

FAB
fast atom bombardment
MS MS
nucleosides
tandem mass spectrometry

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10.1016/0003-2697(84)90412-3

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@article{f2bf98d45b2e44c2a63eb2bbe3651938,

title = "Fast atom bombardment combined with tandem mass spectrometry for the determination of nucleosides",

abstract = "The positive and negative ion fast atom bombardment (FAB) mass spectra and fast atom bombardment collisionally activated decomposition (CAD) spectra of a series of nucleosides and two dinucleotides are reported. The nucleosides studied are substituted forms of guanosine, adenosine, nebularine, tubercidin, uridine, and related pyrimidines. The FAB and CAD data both contain similar information. The CAD spectra are found to provide some structural information not found in the FAB mass spectra. Tandem mass spectrometry also allows emphasis to be put on weak fragments which are either not observed in the FAB mass spectrum or are lost in the matrix ion signals.",

keywords = "FAB, fast atom bombardment, MS MS, nucleosides, tandem mass spectrometry",

author = "Crow, \{Frank W.\} and Tomer, \{Kenneth B.\} and Gross, \{Michael L.\} and McCloskey, \{James A.\} and Bergstrom, \{Donald E.\}",

note = "Funding Information: {\textquoteright} This work was supported by the Midwest Center for Mass Spectrometry, a National Science Foundation Regional Instrumentation Facility (Grant CHE 82 I 1164), and by the National Institutes of Health (Grant GM 2 1584 to J.A.M.). * Abbreviations used: FAB, fast atom bombardment; EI, electron ionization; CI, chemical ionization; MS/MS, tandem mass spectrometry; GC/MS, gas chromatography/ mass spectrometry; CA, collisional activation; CAD, col-lisionahy activated decomposition; G, glycerol; B, base.",

year = "1984",

month = may,

day = "15",

doi = "10.1016/0003-2697(84)90412-3",

language = "English",

volume = "139",

pages = "243--262",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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TY - JOUR

T1 - Fast atom bombardment combined with tandem mass spectrometry for the determination of nucleosides

AU - Crow, Frank W.

AU - Tomer, Kenneth B.

AU - Gross, Michael L.

AU - McCloskey, James A.

AU - Bergstrom, Donald E.

N1 - Funding Information: ’ This work was supported by the Midwest Center for Mass Spectrometry, a National Science Foundation Regional Instrumentation Facility (Grant CHE 82 I 1164), and by the National Institutes of Health (Grant GM 2 1584 to J.A.M.). * Abbreviations used: FAB, fast atom bombardment; EI, electron ionization; CI, chemical ionization; MS/MS, tandem mass spectrometry; GC/MS, gas chromatography/ mass spectrometry; CA, collisional activation; CAD, col-lisionahy activated decomposition; G, glycerol; B, base.

PY - 1984/5/15

Y1 - 1984/5/15

N2 - The positive and negative ion fast atom bombardment (FAB) mass spectra and fast atom bombardment collisionally activated decomposition (CAD) spectra of a series of nucleosides and two dinucleotides are reported. The nucleosides studied are substituted forms of guanosine, adenosine, nebularine, tubercidin, uridine, and related pyrimidines. The FAB and CAD data both contain similar information. The CAD spectra are found to provide some structural information not found in the FAB mass spectra. Tandem mass spectrometry also allows emphasis to be put on weak fragments which are either not observed in the FAB mass spectrum or are lost in the matrix ion signals.

AB - The positive and negative ion fast atom bombardment (FAB) mass spectra and fast atom bombardment collisionally activated decomposition (CAD) spectra of a series of nucleosides and two dinucleotides are reported. The nucleosides studied are substituted forms of guanosine, adenosine, nebularine, tubercidin, uridine, and related pyrimidines. The FAB and CAD data both contain similar information. The CAD spectra are found to provide some structural information not found in the FAB mass spectra. Tandem mass spectrometry also allows emphasis to be put on weak fragments which are either not observed in the FAB mass spectrum or are lost in the matrix ion signals.

KW - FAB

KW - fast atom bombardment

KW - MS MS

KW - nucleosides

KW - tandem mass spectrometry

UR - https://www.scopus.com/pages/publications/0021256035

U2 - 10.1016/0003-2697(84)90412-3

DO - 10.1016/0003-2697(84)90412-3

M3 - Article

C2 - 6331227

AN - SCOPUS:0021256035

SN - 0003-2697

VL - 139

SP - 243

EP - 262

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Fast atom bombardment combined with tandem mass spectrometry for the determination of nucleosides

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Keywords

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