TY - JOUR
T1 - Fast atom bombardment and tandem mass spectrometry for structure determination of steroid and flavonoid glycosides
AU - Crow, Frank W.
AU - Tomer, Kenneth B.
AU - Looker, James H.
AU - Gross, Michael L.
N1 - Funding Information:
’ This work was supported by the Midwest Center for Mass Spectrometry, a National Science Foundation Regional Instrumentation Facility (Grant CHE 8211164). 2 Present address: The Upjohn Co. Unit 7832-259-12. 7000 Portage Rd., Kalamazoo, Mich. 4900 1. 3 To whom correspondence should be addressed. 4 Abbreviations used: FAB, fast atom bombardment; EIMS, electron ionization mass spectrometry; DCI. desorption chemical ionization; FD, field desorption; SIMS, secondary ion mass spectrometry; CA, collisional activation; CAD, collisionally activated decomposition: NICI, negative-ion-chemical ionization.
PY - 1986/6
Y1 - 1986/6
N2 - The combination of fast atom bombardment (FAB) and tandem mass spectrometry (MS-MS) was tested for its applicability to generate useful structural information for steroid and flavonoid glycosides. The following compounds were investigated: quercetin, myricitrin, apigetrin, fraxin, rutin, neohesperidin, hesperidin, naringin, apiin, cymarin, digoxin, digitoxin, xanthorhamnin, and frangulin. Upon FAB, the sample molecules are desorbed as (M + H)+, (M - H)-, or as (M + Na)+ or (M + K)+. Collisional activation of (M + H)+ or (M - H)- ions in the MS-MS experiment leads to sequential losses of glycoside moieties in a manner which permits the sequence of glycosides to be established. Some glycosides occur as mixtures of homologs. Proper interpretation of the MS-MS or collisional activation decomposition spectra often allows the homology to be located. In addition to the simple and highly selective fragmentations observed in this combined experiment, FAB and MS-MS also remove interference caused by the ubiquitous matrix ions which are desorbed by FAB.
AB - The combination of fast atom bombardment (FAB) and tandem mass spectrometry (MS-MS) was tested for its applicability to generate useful structural information for steroid and flavonoid glycosides. The following compounds were investigated: quercetin, myricitrin, apigetrin, fraxin, rutin, neohesperidin, hesperidin, naringin, apiin, cymarin, digoxin, digitoxin, xanthorhamnin, and frangulin. Upon FAB, the sample molecules are desorbed as (M + H)+, (M - H)-, or as (M + Na)+ or (M + K)+. Collisional activation of (M + H)+ or (M - H)- ions in the MS-MS experiment leads to sequential losses of glycoside moieties in a manner which permits the sequence of glycosides to be established. Some glycosides occur as mixtures of homologs. Proper interpretation of the MS-MS or collisional activation decomposition spectra often allows the homology to be located. In addition to the simple and highly selective fragmentations observed in this combined experiment, FAB and MS-MS also remove interference caused by the ubiquitous matrix ions which are desorbed by FAB.
KW - FAB
KW - fast atom bombardment
KW - flavonoid glycosides
KW - tandem mass spectrometry, MS MS
UR - http://www.scopus.com/inward/record.url?scp=0022729192&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(86)90438-0
DO - 10.1016/0003-2697(86)90438-0
M3 - Article
C2 - 3728980
AN - SCOPUS:0022729192
SN - 0003-2697
VL - 155
SP - 286
EP - 307
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -