Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans

K. B. Tomer, F. W. Crow, H. W. Knoche, M. L. Gross, H. W. Knoche

Research output: Contribution to journalArticlepeer-review

Abstract

Two thermally labile, zwitterionic ornithine-containing lipids isolated as a mixture from Thiobacillus thiooxidans have been analyzed by using fast atom bombardment (FAB) for vaporization and mass spectrometry/mass spectrometry (MS/MS) for analysis. Each lipid component yielded a gas-phase protonated molecule whose compositions were determined to be C40H77N2O6 and C39H7SN2O6. Collision Induced decomposition (CID) spectra of the lower molecular weight homologue obtained in an MS/MS mode revealed that part of the molecule which has one less methylene group. The molecular mass assignments of the constituents were verified by a study of the (M − H) negative Ions. CID spectra of these negative ions gave new structural Information which was complementary to that from the CID spectra of the positive ions.

Original languageEnglish
Pages (from-to)1033-1036
Number of pages4
JournalAnalytical Chemistry
Volume55
Issue number7
DOIs
StatePublished - 1983

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