Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans

K. B. Tomer; F. W. Crow; H. W. Knoche; M. L. Gross; H. W. Knoche

doi:10.1021/ac00258a013

Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans

K. B. Tomer, F. W. Crow, H. W. Knoche, M. L. Gross, H. W. Knoche

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Abstract

Two thermally labile, zwitterionic ornithine-containing lipids isolated as a mixture from Thiobacillus thiooxidans have been analyzed by using fast atom bombardment (FAB) for vaporization and mass spectrometry/mass spectrometry (MS/MS) for analysis. Each lipid component yielded a gas-phase protonated molecule whose compositions were determined to be C₄₀H₇₇N₂O₆ and C₃₉H_7SN₂O₆. Collision Induced decomposition (CID) spectra of the lower molecular weight homologue obtained in an MS/MS mode revealed that part of the molecule which has one less methylene group. The molecular mass assignments of the constituents were verified by a study of the (M − H) negative Ions. CID spectra of these negative ions gave new structural Information which was complementary to that from the CID spectra of the positive ions.

Original language	English
Pages (from-to)	1033-1036
Number of pages	4
Journal	Analytical Chemistry
Volume	55
Issue number	7
DOIs	https://doi.org/10.1021/ac00258a013
State	Published - 1983

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title = "Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans",

abstract = "Two thermally labile, zwitterionic ornithine-containing lipids isolated as a mixture from Thiobacillus thiooxidans have been analyzed by using fast atom bombardment (FAB) for vaporization and mass spectrometry/mass spectrometry (MS/MS) for analysis. Each lipid component yielded a gas-phase protonated molecule whose compositions were determined to be C40H77N2O6 and C39H7SN2O6. Collision Induced decomposition (CID) spectra of the lower molecular weight homologue obtained in an MS/MS mode revealed that part of the molecule which has one less methylene group. The molecular mass assignments of the constituents were verified by a study of the (M − H) negative Ions. CID spectra of these negative ions gave new structural Information which was complementary to that from the CID spectra of the positive ions.",

author = "Tomer, {K. B.} and Crow, {F. W.} and Knoche, {H. W.} and Gross, {M. L.} and Knoche, {H. W.}",

year = "1983",

doi = "10.1021/ac00258a013",

language = "English",

volume = "55",

pages = "1033--1036",

journal = "Analytical Chemistry",

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T1 - Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans

AU - Tomer, K. B.

AU - Crow, F. W.

AU - Knoche, H. W.

AU - Gross, M. L.

AU - Knoche, H. W.

PY - 1983

Y1 - 1983

N2 - Two thermally labile, zwitterionic ornithine-containing lipids isolated as a mixture from Thiobacillus thiooxidans have been analyzed by using fast atom bombardment (FAB) for vaporization and mass spectrometry/mass spectrometry (MS/MS) for analysis. Each lipid component yielded a gas-phase protonated molecule whose compositions were determined to be C40H77N2O6 and C39H7SN2O6. Collision Induced decomposition (CID) spectra of the lower molecular weight homologue obtained in an MS/MS mode revealed that part of the molecule which has one less methylene group. The molecular mass assignments of the constituents were verified by a study of the (M − H) negative Ions. CID spectra of these negative ions gave new structural Information which was complementary to that from the CID spectra of the positive ions.

AB - Two thermally labile, zwitterionic ornithine-containing lipids isolated as a mixture from Thiobacillus thiooxidans have been analyzed by using fast atom bombardment (FAB) for vaporization and mass spectrometry/mass spectrometry (MS/MS) for analysis. Each lipid component yielded a gas-phase protonated molecule whose compositions were determined to be C40H77N2O6 and C39H7SN2O6. Collision Induced decomposition (CID) spectra of the lower molecular weight homologue obtained in an MS/MS mode revealed that part of the molecule which has one less methylene group. The molecular mass assignments of the constituents were verified by a study of the (M − H) negative Ions. CID spectra of these negative ions gave new structural Information which was complementary to that from the CID spectra of the positive ions.

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Fast Atom Bombardment and Mass Spectrometry/Mass Spectrometry for Analysis of a Mixture of Ornithine-Containing Lipids from Thiobacillus thiooxidans

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