Failure of 1,25 dihydroxycholecalciferol [1,25-(OH)1-d3] to modify cyclic AMP levels in parathyroid hormone-treated and untreated bone cells (1)

William A. Peck, Isabel Dowling

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    16 Scopus citations

    Abstract

    Treatment with 1,25-(OH)2-D3 (1 ng/ml - 25 ng/ml) for periods ranging from 2.5 min. to 60 min. did not alter cyclic AMP levels in bone cells isolated from periosteum-free rat calvaria, or in cells isolated from rat periosteal tissues. 1,25-(OH)2-D3 failed to modify the acute increases in cyclic AMP elicited by PTH (10 ng/ml -1 ug/ml). Two separate 1,25-(OH)2-D3 preparations, biologically active in other systems, were ineffective under a wide variety of experimental conditions. These results suggest that 1,25-(OH)2-D3 is not an acute modulator of cyclic AMP metabolism in PTH-treated and untreated bone cells.

    Original languageEnglish
    Pages (from-to)157-166
    Number of pages10
    JournalEndocrine Research
    Volume3
    Issue number2
    DOIs
    StatePublished - 1976

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