Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

Patricia J. Anderson; David Gailani; Hendrik B. Feys; Weiqiang Gao; Elaine M. Majerus; Karen Vanhoorelbeke; J. Evan Sadler

doi:10.3324/haematol.11478

Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

Patricia J. Anderson
, David Gailani
, Hendrik B. Feys
, Weiqiang Gao
, Elaine M. Majerus
, Karen Vanhoorelbeke
, J. Evan Sadler

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

Original language	English
Pages (from-to)	1419-1422
Number of pages	4
Journal	Haematologica
Volume	92
Issue number	10
DOIs	https://doi.org/10.3324/haematol.11478
State	Published - Oct 2007

Keywords

ADAMTS13
Factor XI
Von Willebrand Factor

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10.3324/haematol.11478

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title = "Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma",

abstract = "Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670\% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.",

keywords = "ADAMTS13, Factor XI, Von Willebrand Factor",

author = "Anderson, \{Patricia J.\} and David Gailani and Feys, \{Hendrik B.\} and Weiqiang Gao and Majerus, \{Elaine M.\} and Karen Vanhoorelbeke and Sadler, \{J. Evan\}",

year = "2007",

month = oct,

doi = "10.3324/haematol.11478",

language = "English",

volume = "92",

pages = "1419--1422",

journal = "Haematologica",

issn = "0390-6078",

number = "10",

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TY - JOUR

T1 - Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

AU - Anderson, Patricia J.

AU - Gailani, David

AU - Feys, Hendrik B.

AU - Gao, Weiqiang

AU - Majerus, Elaine M.

AU - Vanhoorelbeke, Karen

AU - Sadler, J. Evan

PY - 2007/10

Y1 - 2007/10

N2 - Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

AB - Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

KW - ADAMTS13

KW - Factor XI

KW - Von Willebrand Factor

UR - https://www.scopus.com/pages/publications/36348993732

U2 - 10.3324/haematol.11478

DO - 10.3324/haematol.11478

M3 - Article

C2 - 17768109

AN - SCOPUS:36348993732

SN - 0390-6078

VL - 92

SP - 1419

EP - 1422

JO - Haematologica

JF - Haematologica

IS - 10

ER -

Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

Abstract

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