Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

Patricia J. Anderson, David Gailani, Hendrik B. Feys, Weiqiang Gao, Elaine M. Majerus, Karen Vanhoorelbeke, J. Evan Sadler

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Abstract

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

Original languageEnglish
Pages (from-to)1419-1422
Number of pages4
JournalHaematologica
Volume92
Issue number10
DOIs
StatePublished - Oct 1 2007

Keywords

  • ADAMTS13
  • Factor XI
  • Von Willebrand Factor

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    Anderson, P. J., Gailani, D., Feys, H. B., Gao, W., Majerus, E. M., Vanhoorelbeke, K., & Sadler, J. E. (2007). Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma. Haematologica, 92(10), 1419-1422. https://doi.org/10.3324/haematol.11478