TY - JOUR
T1 - Facile separation of enantiomers, geometrical isomers, and routine compounds on stable cyclodextrin LC bonded phases
AU - Armstrong, D. W.
AU - Alak, A.
AU - Bui, K.
AU - Demond, W.
AU - Ward, T.
AU - Riehl, T. E.
AU - Hinze, W. L.
PY - 1984/9/1
Y1 - 1984/9/1
N2 - The effectiveness of employing stationary phases composed of chemically bonded cyclodextrin molecules in the high performance liquid chromatographic separation of a variety of different types of compounds is summarized. Over one hundred compounds, including optical, geometrical, and structural isomers, diastereomers, and epimers were successfully separated from each other via use of beta- or gamma-cyclodextrin bonded phases and aqueous methanolic mobile phases. The mechanism of separation is based upon inclusion complex formation between the compounds being separated and the cyclodextrin molecules bonded to the stationary phase. The effects of temperature, mobile phase composition and flow rate upon the chromatographic selectivity and resolution are described. The results indicate that the cyclodextrin columns may be more versatile, flexible, and effective compared to the conventional normal or reversed phase columns.
AB - The effectiveness of employing stationary phases composed of chemically bonded cyclodextrin molecules in the high performance liquid chromatographic separation of a variety of different types of compounds is summarized. Over one hundred compounds, including optical, geometrical, and structural isomers, diastereomers, and epimers were successfully separated from each other via use of beta- or gamma-cyclodextrin bonded phases and aqueous methanolic mobile phases. The mechanism of separation is based upon inclusion complex formation between the compounds being separated and the cyclodextrin molecules bonded to the stationary phase. The effects of temperature, mobile phase composition and flow rate upon the chromatographic selectivity and resolution are described. The results indicate that the cyclodextrin columns may be more versatile, flexible, and effective compared to the conventional normal or reversed phase columns.
UR - http://www.scopus.com/inward/record.url?scp=0001469420&partnerID=8YFLogxK
U2 - 10.1007/BF00662219
DO - 10.1007/BF00662219
M3 - Article
AN - SCOPUS:0001469420
SN - 0167-7861
VL - 2
SP - 533
EP - 545
JO - Journal of Inclusion Phenomena
JF - Journal of Inclusion Phenomena
IS - 3-4
ER -