Facile production of multivalent enzyme-nanoparticle conjugates

Sam J. Kuhn, Stephanie K. Finch, Dennis E. Hallahan, Todd D. Giorgio

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Nanoparticles (NPs) enable the development of new, biologically relevant, multifunctional agents. Multifunctional NPs can mimic the invasive biological processes involved in cancer metastasis. We have demonstrated a facile and controllable method of conjugating multiple enzyme species to superparamagnetic (SPM) NPs. Horseradish peroxidase, α-glucosidase, and collagenase were conjugated to SPM NPs with a functional enzyme:NP ratio of 15:127:103:1. N-succinimidyl-S-acetylthiopropionate (SATP)-mediated addition of sulfhydryls to enzymes was achieved without significant activity loss. Conjugation of all enzymes was simultaneously accomplished using maleimide reactive groups generated from activation of amines associated with polyethylene glycol molecules on the NP surface. Cross reactivity between enzyme activity assay systems was negligible.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalJournal of Magnetism and Magnetic Materials
Volume311
Issue number1 SPEC. ISS.
DOIs
StatePublished - Apr 2007

Keywords

  • Conjugation
  • Enzyme
  • Multifunctional
  • Nanoparticle
  • Superparamagnetic

Fingerprint

Dive into the research topics of 'Facile production of multivalent enzyme-nanoparticle conjugates'. Together they form a unique fingerprint.

Cite this