Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Microsphere-assisted imaging can be incorporated onto conventional light microscopes allowing wide-field and flourescence imaging with enhanced resolution. We demonstrated that imaging of specimens containing subdiffraction-limited features is achievable through high-index microspheres embedded in a transparent thin film placed over the specimen. We fabricated novel microsphere-embedded microscope slides composed of barium titanate glass microspheres (with diameter ∼10-100 μm and refractive index∼1.9-2.2) embedded in a transparent polydimethylsiloxane (PDMS) elastomer layer with controllable thickness. We characterized the imaging performance of such microsphere-embedded devices in white-light microscopies, by measuring the imaging resolution, field-of-view, and magnification as a function of microsphere size. Our results inform on the design of novel optical devices, such as microsphere-embedded microscope slides for imaging applications.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXV
EditorsTony Wilson, Carol J. Cogswell, Thomas G. Brown
PublisherSPIE
ISBN (Electronic)9781510614833
DOIs
StatePublished - 2018
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 - San Francisco, United States
Duration: Jan 29 2018Jan 31 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10499
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018
Country/TerritoryUnited States
CitySan Francisco
Period01/29/1801/31/18

Keywords

  • PDMS
  • Super-resolution
  • barium titanate
  • microscopy
  • microsphere
  • nanoscopy

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