Extrapineal N-acetyltransferase activity in rat brain

Steven M. Paul; Louise L. Hsu; Arnold J. Mandell

doi:10.1016/0024-3205(74)90030-7

Extrapineal N-acetyltransferase activity in rat brain

Steven M. Paul
, Louise L. Hsu
, Arnold J. Mandell

School of Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

An extrapineal enzyme that N-acetylates a variety of indole and catechol alkylamines, including the psychoactive drugs mescaline and d-amphetamine, has been partially purified from rat brain. This enzymatic activity has a distinct pH optimum, is linear with incubation time and protein concetration, and is abolished by heating. Purification is approximately ten fold with ammonium sulfate precipitation and column chromatography, yielding a specific activity of 7936 pmoles of product per mg of protein per hour with tryptamine as substrate.

Original language	English
Pages (from-to)	2135-2143
Number of pages	9
Journal	Life Sciences
Volume	15
Issue number	12
DOIs	https://doi.org/10.1016/0024-3205(74)90030-7
State	Published - Dec 15 1974

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title = "Extrapineal N-acetyltransferase activity in rat brain",

abstract = "An extrapineal enzyme that N-acetylates a variety of indole and catechol alkylamines, including the psychoactive drugs mescaline and d-amphetamine, has been partially purified from rat brain. This enzymatic activity has a distinct pH optimum, is linear with incubation time and protein concetration, and is abolished by heating. Purification is approximately ten fold with ammonium sulfate precipitation and column chromatography, yielding a specific activity of 7936 pmoles of product per mg of protein per hour with tryptamine as substrate.",

author = "Paul, \{Steven M.\} and Hsu, \{Louise L.\} and Mandell, \{Arnold J.\}",

note = "Funding Information: This work ie supported by IISPHS Grast DA-00265-03 and vas done while",

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AU - Hsu, Louise L.

AU - Mandell, Arnold J.

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PY - 1974/12/15

Y1 - 1974/12/15

N2 - An extrapineal enzyme that N-acetylates a variety of indole and catechol alkylamines, including the psychoactive drugs mescaline and d-amphetamine, has been partially purified from rat brain. This enzymatic activity has a distinct pH optimum, is linear with incubation time and protein concetration, and is abolished by heating. Purification is approximately ten fold with ammonium sulfate precipitation and column chromatography, yielding a specific activity of 7936 pmoles of product per mg of protein per hour with tryptamine as substrate.

AB - An extrapineal enzyme that N-acetylates a variety of indole and catechol alkylamines, including the psychoactive drugs mescaline and d-amphetamine, has been partially purified from rat brain. This enzymatic activity has a distinct pH optimum, is linear with incubation time and protein concetration, and is abolished by heating. Purification is approximately ten fold with ammonium sulfate precipitation and column chromatography, yielding a specific activity of 7936 pmoles of product per mg of protein per hour with tryptamine as substrate.

UR - https://www.scopus.com/pages/publications/0016394353

U2 - 10.1016/0024-3205(74)90030-7

DO - 10.1016/0024-3205(74)90030-7

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JO - Life Sciences

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ER -

Extrapineal N-acetyltransferase activity in rat brain

Abstract

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