Abstract
An extrapineal enzyme that N-acetylates a variety of indole and catechol alkylamines, including the psychoactive drugs mescaline and d-amphetamine, has been partially purified from rat brain. This enzymatic activity has a distinct pH optimum, is linear with incubation time and protein concetration, and is abolished by heating. Purification is approximately ten fold with ammonium sulfate precipitation and column chromatography, yielding a specific activity of 7936 pmoles of product per mg of protein per hour with tryptamine as substrate.
Original language | English |
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Pages (from-to) | 2135-2143 |
Number of pages | 9 |
Journal | Life Sciences |
Volume | 15 |
Issue number | 12 |
DOIs | |
State | Published - Dec 15 1974 |