Extragenic suppressors of the nimX2(cdc2) mutation of Aspergillus nidulans affect nuclear division, septation and conidiation

S. L. McGuire, D. L. Roe, B. W. Carter, R. L. Carter, S. P. Grace, P. L. Hays, G. A. Lang, J. L.C. Mamaril, A. T. McElvaine, A. M. Payne, M. D. Schrader, S. E. Wahrle, C. D. Young

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Abstract

The Aspergillus nidulans NIMX(CDC2) protein kinase has been shown to be required for both the G2/M and G1/S transitions, and recent evidence has implicated a role for NIMX(CDC2) in septation and conidiation. While much is understood of its G2/M function, little is known about the functions of NIMX(CDC2) during G1/S, septation, and conidiophore development. In an attempt to better understand how NIMX(CDC2) is involved in these processes, we have isolated four extragenic suppressors of the A nidulans nimX2(cdc2) temperature-sensitive mutation. Mutation of these suppressor genes, designated snxA-snxD for suppressor of nimX, affects nuclear division, septation, and conidiation. The cold-sensitive snxA1 mutation leads to arrest of nuclear division during G1 or early S. snxB1 causes hyperseptation in the hyphae and sensitivity to hydroxyurea, while snxC1 causes septation in the conidiophore stalk and aberrant conidiophore structure snxD1 leads to slight septation defects and hydroxyurea sensitivity. The additional phenotypes that result from the suppressor mutations provide genetic evidence that NIMX(CDC2) affects septation and conidiation in addition to nuclear division, and cloning and biochemical analysis of these will allow a better understanding of the role of NIMX(CDC2) in these processes.

Original languageEnglish
Pages (from-to)1573-1584
Number of pages12
JournalGenetics
Volume156
Issue number4
StatePublished - Dec 1 2000
Externally publishedYes

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    McGuire, S. L., Roe, D. L., Carter, B. W., Carter, R. L., Grace, S. P., Hays, P. L., Lang, G. A., Mamaril, J. L. C., McElvaine, A. T., Payne, A. M., Schrader, M. D., Wahrle, S. E., & Young, C. D. (2000). Extragenic suppressors of the nimX2(cdc2) mutation of Aspergillus nidulans affect nuclear division, septation and conidiation. Genetics, 156(4), 1573-1584.