Abstract
We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 m guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.
| Original language | English |
|---|---|
| Pages (from-to) | 89-95 |
| Number of pages | 7 |
| Journal | Analytical Biochemistry |
| Volume | 202 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 1992 |
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Adams, M. E., Qiu Huang, D., Yue Yao, L., & Sandell, L. J. (1992). Extraction and isolation of mRNA from adult articular cartilage. Analytical Biochemistry, 202(1), 89-95. https://doi.org/10.1016/0003-2697(92)90211-O