Extraction and isolation of mRNA from adult articular cartilage

Mark E. Adams, Ding Qiu Huang, Lena Yue Yao, Linda J. Sandell

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 m guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalAnalytical Biochemistry
Volume202
Issue number1
DOIs
StatePublished - Apr 1992

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