TY - JOUR
T1 - Extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase
AU - Hall, Kathleen
AU - Cruz, Phillip
AU - Chamberlin, Michael J.
N1 - Funding Information:
‘This work was supported in part by National Institutes of Health Grants GM10840 (I. Tinoco, Jr.) GM12010 (M.J.C.), and U. S. Department of Energy Office of Energy Research, under Contract 03-82ER60090.000 (I.T.). * To whom correspondence should be addressed.
PY - 1985/1
Y1 - 1985/1
N2 - Conditions have been found that allow for extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase with a poly[d(I-C)]·poly[d(I-C)] template. The extensive synthesis, in which many copies of the template were produced by the enzyme, continued for 24 h. Repeated addition of the template was necessary for synthesis to continue, as the poly[r(G-C)] appeared to be binding to the template, inhibiting transcription by the polymerase. Four hundred ODU, or 20 mg of the poly[r(G-C)] has been prepared using 0.3 mg of protein.
AB - Conditions have been found that allow for extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase with a poly[d(I-C)]·poly[d(I-C)] template. The extensive synthesis, in which many copies of the template were produced by the enzyme, continued for 24 h. Repeated addition of the template was necessary for synthesis to continue, as the poly[r(G-C)] appeared to be binding to the template, inhibiting transcription by the polymerase. Four hundred ODU, or 20 mg of the poly[r(G-C)] has been prepared using 0.3 mg of protein.
UR - http://www.scopus.com/inward/record.url?scp=0021901508&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(85)90604-6
DO - 10.1016/0003-9861(85)90604-6
M3 - Article
C2 - 3881085
AN - SCOPUS:0021901508
SN - 0003-9861
VL - 236
SP - 47
EP - 51
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -