Extending microsphere-assisted microscopy to polarization-driven dynamic laser speckle analysis

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Abstract

Polarimetric imaging is a powerful optical tool for probing material properties by analyzing light polarization effects. However, conventional polarimetric image analysis struggles with addressing complex polarization scenarios due to limitations in disentangling intertwined polarization effects and the detrimental impact of laser-induced speckle. Previously, we proposed combining polarimetric imaging with dynamic laser speckle analysis (DLSA) to overcome these challenges. This synergistic approach focuses on changes in speckle pattern across intensity images acquired during a full polarimetry measurement. Here, we extend this technique into the microscopy domain and enhance the lateral resolution by incorporating microsphere-assisted microscopy (MAM). The integration of these three techniques into a single setup offers a comprehensive approach for applications requiring high-resolution polarimetric analysis.

Original languageEnglish
Title of host publicationLabel-Free Biomedical Imaging and Sensing (LBIS) 2025
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510684102
DOIs
StatePublished - 2025
EventLabel-Free Biomedical Imaging and Sensing, LBIS 2025 - San Francisco, United States
Duration: Jan 26 2025Jan 29 2025

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume13331
ISSN (Print)1605-7422

Conference

ConferenceLabel-Free Biomedical Imaging and Sensing, LBIS 2025
Country/TerritoryUnited States
CitySan Francisco
Period01/26/2501/29/25

Keywords

  • Dynamic laser speckle analysis
  • Microsphere-assisted microscopy
  • Numerical aperture
  • Polarimetric imaging
  • Super-resolution

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