Expression, purification, and functional characterization of the insulin-responsive facilitative glucose transporter GLUT4

Keyphrases

• Insulin 100%
• Glucose Transporter 4 (GLUT4) 100%
• Facilitative Glucose Transporter 100%
• Functional Characterization 100%
• Expression Characterization 100%
• Purification Characterization 100%
• Reconstitution 18%
• Biophysical Analysis 18%
• Maltose 18%
• Proteoliposomes 18%
• Neopentyl Glycol 18%
• Glucose Homeostasis 9%
• Overexpression 9%
• Carboxyl Terminus 9%
• Highly Effective 9%
• Ligand Binding 9%
• HEK293 Cells 9%
• His-tag 9%
• Structural Integrity 9%
• Enhanced Green Fluorescent Protein (eGFP) 9%
• Size Exclusion Chromatography 9%
• Amino-terminus 9%
• Biochemical Analysis 9%
• Fluorescence Detection 9%
• Photolabeling 9%
• Extended Period 9%
• Functional Activity 9%
• Tryptophan Fluorescence 9%
• Cytochalasin B 9%
• L-glucose 9%
• Saturable 9%
• Elevated Temperature 9%
• D-glucose 9%
• Detergent Solubilized 9%
• FLAG Tag 9%
• Suspension Culture 9%
• Intact Protein 9%
• Lipid Nanodiscs 9%
• Systematic Screening 9%
• Detergent Micelles 9%
• A8-35 9%

Biochemistry, Genetics and Molecular Biology

• GLUT4 100%
• Copurification 100%
• Glucose Transporter 100%
• Maltose 18%
• Micelle 18%
• Glucose Homeostasis 9%
• C-Terminus 9%
• Lipid 9%
• N-Terminus 9%
• Ligand Binding 9%
• Size-Exclusion Chromatography 9%
• Photoaffinity Labeling 9%
• HEK 293 Cells 9%
• L-Glucose 9%
• Suspension Cell Culture 9%
• Tryptophan 9%
• Chimeric Protein 9%