Expression profiling of constitutive mast cells reveals a unique identity within the immune system

Daniel F. Dwyer, Nora A. Barrett, K. Frank Austen, Edy Y. Kim, Michael B. Brenner, Laura Shaw, Bingfei Yu, Ananda Goldrath, Sara Mostafavi, Aviv Regev, Andrew Rhoades, Devapregasan Moodley, Hideyuki Yoshida, Diane Mathis, Christophe Benoist, Tsukasa Nabekura, Viola Lam, Lewis L. Lanier, Brian Brown, Miriam MeradViviana Cremasco, Shannon Turley, Paul Monach, Michael L. Dustin, Yuesheng Li, Susan A. Shinton, Richard R. Hardy, Tal Shay, Yilin Qi, Katelyn Sylvia, Joonsoo Kang, Keke Fairfax, Gwendalyn J. Randolph, Michelle L. Robinette, Anja Fuchs, Marco Colonna

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.

Original languageEnglish
Pages (from-to)878-887
Number of pages10
JournalNature immunology
Volume17
Issue number7
DOIs
StatePublished - Jun 21 2016

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    Dwyer, D. F., Barrett, N. A., Austen, K. F., Kim, E. Y., Brenner, M. B., Shaw, L., Yu, B., Goldrath, A., Mostafavi, S., Regev, A., Rhoades, A., Moodley, D., Yoshida, H., Mathis, D., Benoist, C., Nabekura, T., Lam, V., Lanier, L. L., Brown, B., ... Colonna, M. (2016). Expression profiling of constitutive mast cells reveals a unique identity within the immune system. Nature immunology, 17(7), 878-887. https://doi.org/10.1038/ni.3445